Fig. S2.

Expression of lactase and threhalase mRNAs in taste cells. In situ hybridization to taste bud-containing tissues from FNG, FOL, and CV papillae, VEG, and positive control tissue was carried out with digoxigenin-labeled RNA probes for Lct (A–E) and Treh (F–J). Taste cell hybridization to antisense probes indicated expression of mRNAs for all three enzymes in FNG, FOL, and CV taste cells (A–C and F–H). For positive control tissues, Lct mRNA (E) and Treh mRNA (J) were examined in the duodenum. Hybridization of sense probe controls in and around taste cells indicative of nonspecific background was generally lower than with corresponding antisense probes. [Scale bars, 20 (A and F) and 40 μm (B–E and G–J).]