Fig. S4.

α-Glucosidases are not expressed in type I taste cells. Double indirect immunofluorescence confocal microscopy of taste bud-containing sections from mouse CV show cross-labeling when donkey Fab fragment is omitted (A), whereas adequate blocking of rabbit IgG is achieved with excess donkey Fab fragment indicating no cross-labeling of secondary antibodies (B). Double indirect immunofluorescence was carried out with antibodies against MGAM (C, green) and SIS (D, green) and a type 1 marker NTPDase2 (red). No double labeling was observed indicating that these two glucosidase enzymes are not expressed in type I taste cells (Merge). [Scale bars, 20 (A and B) and 40 μm (C and D).]