Table S1.

Results of DNA and biomass PO₄ oxygen isotope analyses for 14 1-L batch culture experiments and calculation of the metabolic water component of intracellular water

Microbial strains	T, °C	δ18OH2O, ‰	δ18OPO4, ‰	δ18OH2O,* ‰	1,000 ln α (PO4−H2O)
DNA
P. fluorescens	23	−5.05	17.2	−4.6	21.6
	30	−5.01	16.8	−4.5	21.2
	37	−5.02	14.0	−4.6	18.5
Acinetobacter	23	−5.56	17.9	−4.9	22.7
	30	−5.32	16.1	−4.8	20.7
	37	−5.09	15.5	−4.6	20.0
M. aquaeolei	24	−5.57	17.4	−4.9	22.2
	37	−5.54	13.9	−4.9	18.7
	30	6.30	24.5	3.3	20.9
	37	6.28	22.0	3.3	18.5
	45	6.34	22.8	3.3	19.3
	30	28.75	39.5	18.8	20.2
	37	28.47	38.7	18.6	19.6
	45	28.20	39.3	18.4	20.3
Biomass
P. fluorescens	23	−5.05	17.2	−4.4	21.4
	30	−5.01	16.5	−4.4	20.7
	37	−5.02	15.3	−4.4	19.6
Acinetobacter	23	−5.56	16.6	−4.7	21.2
	30	−5.32	16.1	−4.6	20.6
	37	−5.09	15.9	−4.4	20.2
M. aquaeolei	24	−5.57	16.8	−4.7	21.4
	37	−5.54	15.2	−4.7	20.3
	30	6.30	23.4	2.3	20.8
	37	6.28	22.4	2.3	19.9
	45	6.34	21.1	2.3	18.6
	30	28.75	37.7	15.5	21.6
	37	28.47	35.0	15.4	19.2
	45	28.20	35.3	15.2	19.6

^*The δ¹⁸O_H2O value of intracellular water is recalculated based on the assumption that metabolic water with a δ¹⁸O_H2O value of −3.5‰ accounts for 31% (for DNA) and 41% (for biomass) of intracellular water.