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. 2016 May 11;113(21):5898–5903. doi: 10.1073/pnas.1523975113

Fig. 2.

Fig. 2.

Studying the effect of designer dimeric compounds on miR-96 biogenesis. (A) Effects of designer dimeric compounds (50 nM) with varied spacing between the RNA-binding modules (n = 1 to n = 4) on pri-, pre-, and mature miR-96 levels in MDA-MB-231 TNBC cells. The compound with n = 2, as indicated in the box, is the most potent inhibitor of miR-96 processing and named 3. (B) Effect of 3 on the in vitro processing of pri-miR-96 by Drosha, indicating that the compound binds the desired site. Error bars are the SDs in the measurements. *P < 0.05 as determined by a two-tailed Student t test (n ≥ 3). (C) Compound 3 binds the predicted site as evidenced by protection from S1 nuclease cleavage. Lane T1 indicates cleavage by T1 nuclease under denaturing conditions (cleaves Gs). Lane L indicates a hydrolysis ladder.