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. 2016 May 11;113(21):5898–5903. doi: 10.1073/pnas.1523975113

Fig. 4.

Fig. 4.

Effect of 3 in TNBC and nontumorigenic mammary epithelial breast cells. (A) Effect of 3 on FOXO1 protein expression, because miR-96 silences FOXO1 protein to suppress apoptosis (13). (B) Effects of compounds 1, 2, and 3 (50 nM) on MDA-MB-231 (TNBC) and MCF 10A (nontumorigenic mammary epithelial breast) cells with or without forced overexpression of pri-miR-96. Compound 3 only triggers apoptosis in MDA-MB-231 cells, and this effect is ablated upon exogenous expression of pri-miR-96. (C) Profiling of all miRNAs predicted to target FOXO1 mRNA by using TargetScan (37) in 3-treated MDA-MB-231 cells. Error bars are the SDs in the measurements and in some cases, too small to be seen. *P < 0.05 as determined by a two-tailed Student t test (n ≥ 3). PI, propidium iodide.