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. 2016 May 11;113(21):5898–5903. doi: 10.1073/pnas.1523975113

Fig. 6.

Fig. 6.

Chem-CLIP and C-Chem-CLIP (14, 15) to study the engagement of pri-miR-96 by 3. (A) Structure of the Targaprimir-96-CA-Biotin (4) Chem-CLIP probe and the control compound that lacks RNA-binding modules [2p-CA-Biotin (5)]. (B) In vitro assessment of the compounds in A for reacting with pri-miR-96. (C) Chem-CLIP and C-Chem-CLIP profiling in MDA-MB-231 cells and quantification by qRT-PCR showing that 4 enriches the pri-miR-96 target in the pull-down. Coaddition of 3 (1,000 or 5,000 nM) and 4 (50 nM) depleted the amount of pri-miR-96 in the pull-down. Error bars are the SDs in the measurements. *P < 0.05 as determined by a two-tailed Student t test (n ≥ 3).