Table 1.

Binding of various small molecules to RNAs and a DNA hairpin

Small molecule	RNAC	RNA1	RNA2	RNA3	RNA4	RNA5	DNA hairpin
1	>30	1.3 ± 0.1	9.4 ± 0.1	3.4 ± 0.1	1.3 ± 0.2	7.4 ± 0.7	>30
2	>2	>20	1.5 ± 0.2	2.7 ± 0.3	>20	1.0 ± 0.1	0.2 ± 0.02
Targaprimir-96 (3)	>4	1.2 ± 0.3	0.9 ± 0.2	0.085 ± 0.01; 0.020 ± 0.007*	1.2 ± 0.3	1.5 ± 0.2	>50

K_d values are reported in micromolar.

^*This measurement was completed in the presence of PEG 8000, a molecular crowding agent that allows in vitro conditions to mimic the molecularly crowded environment in cells.