Fig. S2.

HIF-1α knockdown in COS7 and C2C12 cells by CRISPR/Cas9. pSpCas9(BB)-2A-Puro (PX459) plasmids, which contain a guiding RNA sequence for green monkey or mouse HIF-1α, were transfected to COS7 cells or C2C12 cells. After selection by Puromycin, cells were expanded. To analyze the difference of HIF-1α protein expression in control plasmid-transfected cells and gRNA-inserted plasmid-transfected cells, HIF-1α was inducted by CoCl2 in COS7 cells (A) or C2C12 cells (B), and levels of protein were analyzed by Western blotting.