Fig. S2.

(A) Fittings by two- and three-state HMMs. (B) Dwell time analysis by the three-state HMM. (Left) The dwell time of the highest FRET state among the three states was fitted by single-exponential function of which the rate constant was 1.8 s⁻¹, which was consistent with 2.1 s⁻¹ for the high FRET state in the two-state HMM (main text). (Right) The combined dwell time of the remaining two FRET states was fitted by a convolution of two exponential functions of which the rate constant was 2.5 s⁻¹, which is consistent with 2.4 s⁻¹ for the middle FRET state in the two-state HMM (main text). AIC, Akaike Information Criterion.