Fig. S1.

Secondary structure of NcLPMO9C in solution, as derived from secondary chemical shifts. Secondary chemical shifts allow estimation of dihedral angles, which in turn can be used to predict secondary structure propensities, which are indicated by the blue bars. The pink bars indicate secondary structure assignments based on the crystal structure, and comparison shows that there is excellent agreement between the secondary structure elements observed in solution and those seen in the X-ray crystallographic structure (PDB ID: 4D7U) (26). Such high similarity is not unexpected and has previously been shown to be valid for another LPMO, CBP21, for which both the NMR (PDB ID: 2LHS) (9) and the X-ray (PDB ID: 2BEM) (52) structures are available.