Fig. S2.
The 15N relaxation data and 15N-{1H} heteronuclear NOEs for apo-NcLPMO9C. (A) Longitudinal relaxation time/transverse relaxation time (T1/T2). The T1:T2 ratio is a direct measure of the correlation time for overall rotational tumbling of the protein. The average T1:T2 ratio for NcLPMO9C was calculated to be 13.8 ± 0.7 (SD), which, assuming a spherical particle, corresponds to an overall rotational correlation time, τc, of 11.8 ± 0.5 ns (SD) (44), indicating a tightly packed structure. The Northeast Structural Genomics Consortium has a database with NMR-determined correlation times for globular proteins with different molecular weights. The protein WR73 (which is a 21.9-kDa globular protein) has a longer τc = 13.0 ns. The database is available online at www.nmr2.buffalo.edu/nesg.wiki/NMR_determined_Rotational_correlation_time (accessed March 22, 2016). (B) Steady-state 15N-{1H} NOEs measured for the backbone amide nitrogen atoms.