Fig. 6.
cRLC phosphorylation and increased sarcomere length have synergistic effects on myosin head conformation. Calcium dependence of force (A) and BSR-cRLC-BC probe orientation (B) before (●) and after (○) incubation with cMLCK at 2.3-μm sarcomere length. Maximal isometric force before (black bars) and after (open bars) cMLCK treatment shown in the Inset in A. Dashed and dotted lines represent the Hill equation fits before and after cMLCK incubation, respectively, at sarcomere length 1.9 μm; data from Fig. 2. Means ± SEM (n = 5).