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ERAD substrates interaction with Hrd1 in absence of Hrd3. (A) CPY*HA and Hrd1-5myc interaction was analyzed by co-IP. The IP was analyzed for presence of Hrd1-5myc. The output ratios were calculated by fluorChemE and alphaviewQ from three different experiments. (B) Interaction between Hmg2-GFP and Hrd1-5myc as in Fig. 3A.
