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Hrd3 dependence of ERAD substrate ubiquitination. (A) Hmg2-GFP ubiquitination was assayed in the indicated strains by IP. The percentage intensities (RI%) were quantified by fluorChemE and alphaviewQ from three different experiments. (B) Prd5* ubiquitination was assayed as described in Fig. 4A by using anti-HA IP.
