Fig. 6.

Hrd3 requirement in multimerization and E2 recruitment. (A) Hrd1-Flag and Hrd1-5myc interaction was tested by co-IP. The IP was analyzed for presence of Hrd1-Flag. The output ratios were calculated by fluorChemE and alphaviewQ from three different experiments. (B) Analysis of cross-linking between Hmg2-GFP and Ubc7-2HA. Spheroplasts were harvested from DSP-treated isogenic strains and subjected to an anti-GFP IP, followed by IB for GFP and HA. (C) The cross-linking between Hrd1-5myc and Ubc7-2HA was tested as in Fig. 6B.