Fig. S2.

Overexpression of Hrd3 did not alter Hrd1–substrate interaction. The interaction between Hmg2-GFP and TDH3-driven Hrd1-5myc was tested by co-IP. Microsomes were isolated from logarithmically growing cells and subjected to an anti-GFP IP. The immunoprecipitate was analyzed by SDS/PAGE and immunoblotting for presence of Hrd1 using myc antibodies. Arrows indicate expression from strong TDH3 promoter of the respective genes.