Table S2.
Plasmids used in this study
| Plasmid name | Description | Source |
| pMASTER-lacCONS-N7 | TSS-region library containing 16,295 (∼99.5%) of a possible 16,384 sequences at positions 4- to 10-bp downstream of the −10 element of the lacCONS promoter | (11) |
| pRL706 | Used for overexpression and purification of RNAP-βWT | (34) |
| pRL706-βD446A | Used for overexpression and purification of RNAP-βD446A | (16) |
| pRL706-βWT;3xFLAG | Used for overexpression of 3×FLAG tagged RNAP-βWT | (16) |
| pRL706-βD446A;3xFLAG | Used for overexpression of 3×FLAG tagged RNAP-βD446A | (16) |
| pBEN 493 | Plasmid derived from pACYC184; inserts can be cloned upstream of the tR' terminator on a HindIII, BamHI fragment | (35) |
| pHV-S01 | pBEN493 carrying lacCONS promoter derivative with CGCTGAT TSS- region sequence (in italics) between the HindIII site and BamHI site (used for experiments shown in Fig. 3B). Sequence of insert: AAGCTTGTTCAGAGTTCTACAGTCCGACGATCaggcTTGACActttatgcttcggctcgTATAATgtgCGCTGATgtgagcggataacaatGGATCC | Present work |
| pHV-S02 | pBEN493 carrying lacCONS promoter derivative with CGCTAAT TSS-region sequence between the HindIII site and BamHI site (used for experiments shown in Fig. 3B). Sequence of insert: AAGCTTGTTCAGAGTTCTACAGTCCGACGATCaggcTTGACActttatgcttcggctcgTATAATgtgCGCTAATgtgagcggataacaatGGATCC | Present work |
| pHV-S03 | pBEN493 carrying lacCONS promoter derivative with CGCTCAT TSS-region sequence between the HindIII site and BamHI site (used for experiments shown in Fig. 3B). Sequence of insert: AAGCTTGTTCAGAGTTCTACAGTCCGACGATCaggcTTGACActttatgcttcggctcgTATAATgtgCGCTCATgtgagcggataacaatGGATCC | Present work |
| pHV-S04 | pBEN493 carrying lacCONS promoter derivative with CGCTTAT TSS-region sequence between HindIII and BamHI sites (used for experiments shown in Fig. 3B). Sequence of insert: AAGCTTGTTCAGAGTTCTACAGTCCGACGATCaggcTTGACActttatgcttcggctcgTATAATgtgCGCTTATgtgagcggataacaatGGATCC | Present work |
| pHV-S05 | pBEN493 carrying lacCONS promoter derivative with AACGGCA TSS-region sequence between the HindIII site and BamHI site (used for experiments shown in Fig. 3A). Sequence of insert: AAGCTTGTTCAGAGTTCTACAGTCCGACGATCaggcTTGACActttatgcttcggctcgTATAATgtgAACGGCAgtgagcggataacaatGGATCC | Present work |
| pHV-S06 | pBEN493 carrying lacCONS promoter derivative with AACGACA TSS-region sequence between the HindIII site and BamHI site (used for experiments shown in Fig. 3A). Sequence of insert: AAGCTTGTTCAGAGTTCTACAGTCCGACGATCaggcTTGACActttatgcttcggctcgTATAATgtgAACGACAgtgagcggataacaatGGATCC | Present work |
| pHV-S07 | pBEN493 carrying lacCONS promoter derivative with AACGCCA TSS-region sequence between the HindIII site and BamHI site (used for experiments shown in Fig. 3A). Sequence of insert: AAGCTTGTTCAGAGTTCTACAGTCCGACGATCaggcTTGACActttatgcttcggctcgTATAATgtgAACGCCAgtgagcggataacaatGGATCC | Present work |
| pHV-S08 | pBEN493 carrying lacCONS promoter derivative with AACGTCA TSS region sequence between the HindIII site and BamHI site (used for experiments shown in Fig. 3A). Sequence of insert: AAGCTTGTTCAGAGTTCTACAGTCCGACGATCaggcTTGACActttatgcttcggctcgTATAATgtgAACGTCAgtgagcggataacaatGGATCC | Present work |
| pHV-S17 | pBEN493 carrying PsecE sequence between the HindIII site and BamHI site (used for experiments shown in Fig. 5C). Sequence of insert (TSS positions are capitalized): AAGCTTctttttgcacgctttcgtaccagaacctggctcatcagtgattttctttgtcataatcattgctgagacaggctctgttgagggcgtataatccgaaaAGctaatacgcgtttcGGATCC | Present work |
| pHV-S18 | pBEN493 carrying PhemC sequence between the HindIII site and BamHI site (used for experiments shown in Fig. 5C). Sequence of insert (TSS positions are capitalized): AAGCTTtcaggatccactgccagacctcattttacggtttgcgcaggcgtctacgtttcaccacaacactgacatcactctggcaaggatgttaggatggaccAcGgatgataatgacggGGATCC | Present work |