HV100 |
HindIII upstream primer |
5′-TATAAAGCTTGTTCAGAGTTCTACAGTCCGACGA-3′ |
HV101 |
BamHI downstream primer |
5′-TGCCGGATCCattgttatccgctcacATCAGCGcacATTATAcgagccga-3′ |
HV102 |
BamHI downstream primer |
5′-TGCCGGATCCattgttatccgctcacATTAGCGcacATTATAcgagccga-3′ |
HV103 |
BamHI downstream primer |
5′-TGCCGGATCCattgttatccgctcacATGAGCGcacATTATAcgagccga-3′ |
HV104 |
BamHI downstream primer |
5′-TGCCGGATCCattgttatccgctcacATAAGCGcacATTATAcgagccga-3′ |
HV105 |
BamHI downstream primer |
5′-TGCCGGATCCattgttatccgctcacTGCCGTTcacATTATAcgagccga-3′ |
HV106 |
BamHI downstream primer |
5′-TGCCGGATCCattgttatccgctcacTGTCGTTcacATTATAcgagccga-3′ |
HV107 |
BamHI downstream primer |
5′-TGCCGGATCCattgttatccgctcacTGGCGTTcacATTATAcgagccga-3′ |
HV108 |
BamHI downstream primer |
5′-TGCCGGATCCattgttatccgctcacTGACGTTcacATTATAcgagccga-3′ |
HV117 |
−100 HindIII upstream primer, secE
|
5′-TATAAAGCTTctttttgcacgctttcgtaccag-3′ |
HV118 |
+15 BamHI downstream primer, secE
|
5′-TGCCGGATCCgaaacgcgtattagcttttcg-3′ |
HV119 |
−100 HindIII upstream primer, hemC
|
5′-TATAAAGCTTtcaggatccactgccagacctc-3′ |
HV120 |
+15 BamHI downstream primer, hemC
|
5′-TGCCGGATCCccgtcattatcatccgtggt-3′ |
HV121 |
Biotinylated upstream primer for making IVT templates (anneals 35 base pairs upstream of HindIII site in BN493) |
5′-/5Biosg/GTTGTAATTCTCATGTTTGACAGC-3′ |
HV122 |
Downstream primer for making IVT templates |
5′-GGTCCTCGCCGAAAATGACCCAG-3′ |
HV123 |
For primer extension analyses of RNAs produced from promoter-tR' fusions |
5′-CCTCTCTGCCGGATCC-3′ |
BN436 |
Sequencing primer |
5′-GATTTCAGTGCAATTTATCTC-3′ |
s1086 |
Illumina RA5+6N |
5′-GUUCAGAGUUCUACAGUCCGACGAUCNNNNNN-3′(all bases are RNA)
|
s1082 |
Illumina RTP+9N |
5′-GCCTTGGCACCCGAGAATTCCANNNNNNNNN-3′ |
Illumina RP1 |
|
5′-AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGA-3′ |
Illumina RPI1 |
|
5′-CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA-3′ |
s1206 |
Illumina RA5+15N |
5′-GUUCAGAGUUCUACAGUCCGACGAUCNNNNNNNNNNNNNNN-3′(all bases are RNA)
|
s128 |
RT primer used for generation of MASTER libraries |
5′-CCTTGGCACCCGAGAATTCC-3′ |
s1115 |
Custom primer used for Illumina sequencing |
5′-CTACACGTTCAGAGTTCTACAGTCCGACGATC-3′ |