Fig. S1.

Immunolabeling of excitatory and inhibitory neurons with specific markers. (A-1 and A-2) Hippocampal neurons were fixed at DIV 10 and immunostained against vGlut1 (A-1: an excitatory neuron maker) as well as MAP2 (A-2: green) and βII spectrin (A-2: red). The specificity of vGlut1 staining is evident from the fluorescent intensity contrast observed between the arrow-marked neurons and other neurons in the field of view (A-1). (B-1 and B-2) Similar to A-1 and A-2 except that the neurons were immunostained against parvalbumin, an inhibitory neuron marker. (C-1 and C-2) Similar to A-1 and A-2 except the neurons were immunostained against GAD2, an inhibitory neuron marker. (Scale bar: 50 µm.)