Fig. S4.

βII spectrin is organized into a periodic structure in both excitatory and inhibitory neurons from mouse midbrain. Cultured neurons from mouse midbrain were immunostained with vGlut1 or GAD2 to label the excitatory and inhibitory neurons, respectively. (A) Reconstructed conventional image of a vGlut1+ midbrain neuron. (A-1 and A-2) 3D STORM images of βII spectrin in an axonal (A-1) and dendritic (A-2) region indicated by arrows in A. (Right panels) Autocorrelation functions of the selected boxed regions in the A-1 and A-2 STORM images. (B, B-1, and B-2) Similar to A, A-1, and A-2 but for a GAD2+ inhibitory neuron. (Scale bar: 20 µm for conventional images and 2 µm for STORM images.)