Table S4.

Sample size, mixed-effects model parameters, and estimated effects for data presented in Fig. 3A, β-galactosidase activity at ZH-86Fb

Sample size					Random effects				Fixed effects			Planned comparisons
Day reps	Construct	N (lines)	Vials per line	Total samples		SD		Unequal variance multiplier		Estimate	SE		t value	df	P
6	Single	4	3	72	Day	5.48	Single	1	(Intercept)	100.38	4.72	(Dup – Single) vs. Single	3.68	6	0.0103
	Dup	4	3	72	Line	7.11	Dup	2.52	Dup	133.58	7.69
					Residual	45.88

Model: lme(betagal_activity ∼ construct, random = list(one1 = pdIdent(∼day-1), one2 = pdIdent(∼line-1)), weights = varIdent(form=∼1|construct)). For details, see the legend of Table S1.