Table S10.

Sample size, mixed-effects model parameters, and estimated effects for data presented in Fig. 3G, β-galactosidase activity at attP40

Sample size					Random effects				Fixed effects			Planned comparisons
Day reps	Construct	N (lines)	Vials per line	Total samples		SD		Unequal variance multiplier		Estimate	SE		t value	df	P
4	Single	4	3	96	Day	2.82	Single	1	(Intercept)	99.61	2.91	(Dup – Single) vs. Single	6.90	6	0.00046
	Dup	4	3	96	Line	4.23	Dup	2.75	Dup	137.10	4.60
					Vial	5.43
					Residual	30.56

Model: lme(betagal_activity ∼ construct, random = list(one1 = pdIdent(∼day-1), one2 = pdIdent(∼line-1), one3 = pdIdent(∼vial-1)), weights = varIdent(form=∼1|construct)). For details, see the legend of Table S1.