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. Author manuscript; available in PMC: 2016 Jun 2.
Published in final edited form as: Cell Rep. 2016 May 19;15(9):1876–1883. doi: 10.1016/j.celrep.2016.04.083

FIGURE 4. IGF2BP3 alters mRNA association with RISC.

FIGURE 4

A. Western blot of Ago2 immunoprecipitation from control (NT) or IGF2BP3-depleted (KD) PANC1 cells. (B–F) RT-qPCR analysis of RNA precipitated with α-Ago2 or nonspecific rabbit IgG (Ago2 RIP or IgG RIP, respectively). Relative quantification for each transcript was performed using 18S rRNA as a reference gene. Statistical significance was estimated for each comparison using an unpaired T-test (* P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001).