Fig. 4.

a In the DOR group, the number of mitochondria in oocytes that were central gathering was higher, while the number of mitochondria that belongs to the diffused distribution were lower compared with those in the control group. Both BCTCR and DHEA treatments reversed these changes. Blue column (a) indicates control cells, while brown column (b) represents cells with cyclophosphamide treatment; light green column (c) indicates cells with BSTCR treatment, while purple column (d) represents cells with DHEA treatments. b The percentage of abnormal spindles in oocytes (×100) was significantly increased in the DOR group compared with the control group, as well as BCTCR and DHEA treatments under DOR background. c Representative images of normal and abnormal spindles in oocytes stained by microtubule (green) merged with the nucleus stained by PI (red ). *P < 0.05