Table 1.
Primers used for qRT-PCR analysis of pre-and post-meiotic gene markers and RT-PCR analysis of the SSC-specific genes
| Gene | Primer sequence (5′–3′) | Annealing temperature (°C) | DNA size (bp) |
|---|---|---|---|
| Mvh(Vasa) | F: GATAATCATTTAGCACAGCCTC R: GTCAACAGATGCAAACACAG |
60 | 149 |
| Itgα-6 | F: CTCAGAATATCAAGCTCCCT R: AAACACTAATAGAGCCAGCA |
60 | 148 |
| Gfrα-1 | F: CTGTGGACTAGCTCGCTCTC R: GACCCGCTTTTAGGGGTTCA |
60 | 130 |
| Itgβ-1 | F: GACATTACTCAGATCCAACCA R: AGGTAGTAGAGATCAATAGGGT |
60 | 115 |
| Oct-4 | F:TGGATCCTCGAACCTGGCTA R: CTCAGGCTGCAAAGTCTCCA | 60 | 129 |
| Plzf | F: CCCGTTGGGGGTCAGCTAGAA R: CTGCAAGGTGGGGCGGTGTAG |
60 | 137 |
| Gapdh | F: CTGCTGGACAAGTGAGTCCC R: CCAAGTACCCTGGCCTCATC |
60 | 125 |
| Scp-1 | F: CGCAAGCTGACCTTGAAGTAG R: AGCCCTTCACACTGTTCGTC |
59 | 97 |
| Tekt-1 | F: GAAGGCCCTGTACCGCAGGC R: CCATGGTCGTCGCCGTCTCG |
61 | 132 |