. 2016 Apr 8;33(6):757–763. doi: 10.1007/s10815-016-0709-1

Table 1.

Acrosome loss in the different spermatozoa groups

Spermatozoa treatments	Without acrosome (%)
Mechanical (piezo pulses)	48/104 (46.2)^a
Chemical (ionomycin)	47/110 (42.7)^b
Chemical (Triton X-100)	100/100 (100)^c
Intact, untreated (CTR-IS)	24/120 (20)

Mechanical treatment indicates PPS; chemical treatments indicate spermatozoa treated with ionomycin for 5 min (ionomycin) or with Triton X-100 for 10 min (Triton X-100). Fisher’s exact test

^aPPS vs. CTR-IS, p < 0.0001

^bChemical ionomycin vs. CTR-IS, p < 0.0001

^cChemical Triton X-100 vs. CTR-IS, p < 0.0002