Table 2.
In vitro development of ICSI embryos
| Injected spermatozoa | Oocytes | 2 PN (%) | 2 cells (%) | Blastocysts (%) | |
|---|---|---|---|---|---|
| CTR-IS | Group A | 125 | 10/20 (50) | 13/112 (11.6) | 2/112 (1.8) |
| Group NA | 101 | 9/20 (45) | 9/89 (10.1) | 1/89 (1.1) | |
| PPS | 110 | 22/26 (84.6)a,b | 44/84 (52.3)c | 13/84 (15.5)d | |
| PPS-I | 64 | / | / | 10/64 (15.6) | |
| PPS-T | 70 | / | / | 0/70e | |
Fisher’s exact test
Abbreviations: PPS piezo-pulsed spermatozoa, PPS-I piezo-pulsed spermatozoa pre-treated with ionomycin, PPS-T piezo-pulsed spermatozoa pre-treated with Triton X-100, CTR-IS intact and untreated spermatozoa, Group A activated oocytes, Group NA non-activated oocytes
aPPS vs. Group NA, p = 0.0099
bPPS vs. Group A, p = 0.0221
cPPS vs. Group A, p < 0.0001
dPPS vs. Group A, p = 0.0005
ePPS-T vs. PPS, p = 0.0003