Figure 4.
Neutrophil Fpr2/3−/− is key for NPAs in I/R injury. Wild type (WT, C57Bl/6) and Fpr2/3−/− mice were subjected to MCAo for 60 min, followed by 24 h reperfusion. A) Plasma TXB2 levels were measured. B) representative image of NPAs in cerebral I/R as seen with confocal intravital microscopy (Neutrophil: red; platelets: green). C) neutrophil/platelet aggregates (NPAs) were quantified in sham and I/R WT and Fpr2/3−/− mice. D) Adoptive transfer experiments were performed by injecting CFSE-labeled platelets from donor mice (P) into recipient mice (i.e. KO-P = platelets isolated from Fpr2/3−/− donor mice), and assessed via intravital microscopy. Data are mean ± SEM of 6–8 mice per group. ***p < 0.001 vs. sham. ###p < 0.001 vs. control