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. Author manuscript; available in PMC: 2016 Aug 29.
Published in final edited form as: Leukemia. 2016 Feb 29;30(6):1327–1334. doi: 10.1038/leu.2016.39

Figure 2. MIM-/- cells had increased in expression of CXCR4 on the surface.

Figure 2

(A) Freshly isolated BM cells were stained by PE-CXCR4 antibody and subsequently analyzed by flow cytometry. (B) BM cells were treated with SDF-1 for 10 min at concentrations as indicated, and then analyzed by flow cytometry for the surface expression of CXCR4. All the data represent mean ± SEM (n=3). (C) BM MNCs were stained with antibody against either CXCR4 or CD45 with or without SDF-1 treatment and examined by confocal microscopy. Bar, 2 μm. (D) Quantification of BM MNCs that showed CXCR4 staining mainly at peripheral areas.