Skip to main content
. Author manuscript; available in PMC: 2016 Aug 29.
Published in final edited form as: Leukemia. 2016 Feb 29;30(6):1327–1334. doi: 10.1038/leu.2016.39

Figure 6. p38 antagonist inhibited the increased mobility and the homing activity of MIM-/- cells.

Figure 6

(A) MIM-/- and WT BM cells were treated for 2h with SB203580 at the concentrations as indicated and then analyzed for the level of phosphorylated p38 by Western blot. (B) WT and MIM-/- BM cells were treated with 5 μM SB203580 for 1h and analyzed for the motility response to SDF-1. The data represent mean ± SEM (n=3). (C) WT and MIM-/- BM cells were treated with 5 μM SB203580 for 1h and subsequently transplanted into lethally irradiated mice. After 24h, donor cells were isolated from the BM of recipients and analyzed for the clonogenic activity (n=2). The number of colonies was also compared between treated and non-treated cells and presented as fold decreases (D). (E) BM cells derived from WT and MIM-/- mice were treated with or without 5 μM SB203580 for 1h and then analyzed for the clonogenic activity. The data represents mean ± SEM (n=3). All the p values were based on t-test. NS, no significance.