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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Mucosal Immunol. 2015 Dec 2;9(4):1065–1075. doi: 10.1038/mi.2015.124

Figure 5. Tetherin restricts local HSV-1 spread and neuroinvasion in vivo.

Figure 5

Corneas of WT mice were transfected with scramble or tetherin-specific siRNA, infected, animals euthanized at day 3 or 5 pi, corneas and TG harvested for PCR, 40× confocal imaging, or plaque assay on corneas and TG. (a) Quantification of tetherin expression relative to GAPDH and TBP expression in transfected corneas at 48 and 72 hours pi (n = 5 animals/group/end point; 2 independent experiments; * = p value ≤ 0.05). (b) Representative images of isotype or tetherin-specific antibody labeling with DAPI stain for contrast in the corneal epithelial layers of target or scramble siRNA transfected corneas at day 3 pi (scale bar = 20 µm). (c) Relative siRNA-mediated tetherin protein knockdown shown as images and isometric histogram projections of tetherin MFI from panel b. Imaging was conducted on 3–4 corneas/group; 2–3 independent experiments. (d) HSV-1 titers in individual scramble control and tetherin-specific siRNA transfected mouse corneas (representative data from 1 of 2 experiments shown; n = 6–7 corneas/group total) and TG (n = 8–9 TG/group; 3 independent experiments); *= p < 0.05 comparing scramble siRNA to tetherin siRNA transfected groups.