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. 2016 Jun 2;7:832. doi: 10.3389/fmicb.2016.00832

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Copyright © 2016 Singleton, Graham, Bodman-Smith, Frossard and Steinbach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic analysis of dexa and IL-10 treated MoMØs. Freshly isolated peripheral porcine monocytes were treated with M-CSF for 4 days to obtain MoMØs. MoMØs were treated with dexa (blue) or IL-10 (orange) or left unstimulated (unfilled bars). After a further 24 h MoMØs were harvested and surface stained for pathogen recognition receptors/lineage markers (A) or antigen presentation/co-stimulatory molecules (B) for flow cytometric analysis. Data represent mean percentage of cells expressing markers +SD. One-way ANOVA was used to assess significance followed by Bonferroni’s multiple comparison test ^∗∗∗p < 0.0001, ^∗∗p < 0.001, ^∗p < 0.05.