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. 2016 Jun 2;7:832. doi: 10.3389/fmicb.2016.00832

FIGURE 3.

FIGURE 3

PRRSV replication in MoMØs following different activation stimuli. Monocytes were treated with M-CSF for 4 days to generate MoMØs, and either left unstimulated (red) or activated with LPS and IFN-γ (M1; pink), IL-4 (M2; green), dexa (orange) or IL-10 (blue) for 24 h before infection with PRRSV Lena using an m.o.i of 0.1. RNA was extracted from cells at either 16, 24, 48, or 72 h p.i, and a TaqMan qPCR was used to quantify PRRSV RNA. ΔΔCt represents difference between Ct at 2 h p.i (time zero) and Ct at each time point p.i after normalization against 18S RNA. Bars represent mean ΔΔCt ± SD.