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. 2016 Jun 2;7:832. doi: 10.3389/fmicb.2016.00832

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Copyright © 2016 Singleton, Graham, Bodman-Smith, Frossard and Steinbach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of dexamethasone and IL-10 treatment on the phenotype of porcine MoDCs. Four-day-old MoDCs were treated with either dexamethasone or IL-10 for 24 h before surface staining with various anti-porcine or anti-human mono/poly-clonal antibodies for flow cytometric analysis of surface molecules involved in antigen presentation and maturation (A) or against other co-stimulatory molecules and myeloid lineage markers (B) in untreated MoDCs (unfilled bars), dexa treated MoDCs (blue bars), or IL-10 treated MoDCs (orange bars). Bars show mean percentage of cells expressing each marker +SD (A,B). One-way ANOVA was used to assess significance followed by Bonferroni’s multiple comparison test ^∗∗∗p < 0.0001, ^∗∗p < 0.001, ^∗p < 0.05.