Table 2.
Primers used for qRT-PCR.
| Gene name | Abbreviation | Primer sequence | Amplicon size (bp) | Accession numbers |
|---|---|---|---|---|
| V-Type H+-ATPase a | VHAa | F 5′-GACAAGCACTTCCCAGAGTT-3′ | 134 | ENSGMOT00000017186 |
| R 5′-TCCAGGGTGATCTTATCCGT-3′ | ||||
| V-Type H+-ATPase b | VHAb | F 5′-GCCCTGAACAGAGACATCAA-3′ | 130 | ENSGMOT00000002718 |
| R 5′-GGAGCATCAGCTTGTGTTTG-3′ | ||||
| Na+/K+-ATPase ATP1a1 | ATP1a1 | F 5′-GTCACCATCCTCTGCATTGA-3′ | 132 | ENSGMOT00000005767 |
| R 5′-CCTCTCGTTGACCAGTTTGT-3′ | ||||
| Na+/H+-exchanger 1a | NHE1a | F 5′-CTTCGAGGAGATCCACATCAAC-3′ | 103 | ENSGMOT00000004366 |
| R 5′-CTCGTCAAACAGGTGGTACAG-3′ | ||||
| Na+/H+-exchanger 1b | NHE1b | F 5′-GCGTTCTCTTGATCGTGTTTG-3′ | 137 | ENSGMOT00000013214 |
| R 5′-GGTTCTCACGTGATCGTAGTT-3′ | ||||
| Na+/H+-exchanger 2 | NHE2 | F 5′-CTTTGCCATCTCCTCCATCAT-3′ | 145 | ENSGMOT00000013830 |
| R 5′-GAAGATGAGCGTTTCCGAGAT-3′ | ||||
| Na+/H+-exchanger 3 | NHE3 | F 5′-TGCTGGAGAAGAGCAAGATAAA-3′ | 155 | ENSGMOT00000014326 |
| R 5′-GATGAGCGAGAGATCTGAGTTG-3′ | ||||
| Anion exchanger 1a | AE1a | F 5′-CTTCTTTGCGTTCTGCAAGTC-3′ | 145 | ENSGMOT00000001730 |
| R 5′-CTTGGGTACCATCAACAGGAG-3′ | ||||
| Anion exchanger 1b | AE1b | F 5′-GGTGATGTTGGACTGTAAGGAG-3′ | 128 | ENSGMOT00000001272 |
| R 5′-GGTTGTGGATCAGAGAGTTGAG-3′ | ||||
| Na+/ exchanger 1 | NBC1 | F 5′-CATGAAGAAGTTGCCCAGAGA-3′ | 169 | ENSGMOT00000015497 |
| R 5′-TGGGCCAAGGAGAATGAATAG-3′ | ||||
| Na+/ exchanger 2 | NBC2 | F 5′-CACATCGACTCCCTGAAGATG-3′ | 157 | ENSGMOT00000008943 |
| R 5′-TTGGGATGAACTTGAGGATGG-3′ | ||||
| SLC26a3.1 | A3.1 | F 5′-GATTCCTTCGCCCATCTTCTT-3′ | 151 | ENSGMOT00000014438 |
| R 5′-CCATTCCATGTCTCCGTTCTT-3′ | ||||
| SLC26a3.2 | A3.2 | F 5′-GGAGGTCAATGACACGTACAA-3′ | 143 | ENSGMOT00000014414 |
| R 5′-CCCTCTTCATCTCACCAACAA-3′ | ||||
| SLC26a5 | A5 | F 5′-GAGATCATCGTGGTCATCGT-3′ | 141 | ENSGMOT00000003639 |
| R 5′-GATTCTGGGAAACAGGTGGA-3′ | ||||
| SLC26a6a | A6a | F 5′-CTACCACAAGGTATGGCCTATG-3′ | 105 | ENSGMOT00000019285 |
| R 5′-GGAGGTCCCAAAGATGAAGTAG-3′ | ||||
| SLC26a6b | A6b | F 5′-CCGTGTAGACATGTGTGTGT-3′ | 126 | ENSGMOT00000002392 |
| R 5′-CTTGGTAGCTGGGTCCTAAAG-3 | ||||
| SLC26a6c | A6c | F 5′-GACCCAGCTGCCAACTTATT-3′ | 131 | ENSGMOT00000010364 |
| R 5′-AGTACACCGTGGAAGAGGAA-3′ | ||||
| Carbonic anhydrase 2a | CA2a | F 5′-ACGGACATTCCTTCCAAGTG-3′ | 103 | ENSGMOT00000017293 |
| R 5′-TGGAAGTGGAACTGCTTGAG-3′ | ||||
| Carbonic anhydrase 2b | CA2b | F 5′-GAACGTTCTCTGAAGCCACTTA-3′ | 124 | ENSGMOT00000017257 |
| R 5′-CGGCAGTCAACGTAGAAGTATT-3′ | ||||
| Carbonic anhydrase 4a | CA4a | F 5′-GCACCAAATAGCAACACCAG-3′ | 147 | ENSGMOT00000015251 |
| R 5′-GTGTTCAAACAGGGTCCAGA-3′ | ||||
| Carbonic anhydrase 4b | CA4b | F 5′-CAGTCGCAGGTGTCATGTAA-3′ | 145 | ENSGMOT00000017884 |
| R 5′-TGTAACGGAACCAGCCTTTC-3′ | ||||
| Carbonic anhydrase 4c | CA4c | F 5′-AGTAGCTTGGACGGTGTTTC-3′ | 140 | ENSGMOT00000006568 |
| R 5′-CCAGTAGACAACTCTGCCATC-3′ | ||||
| Carbonic anhydrase 15a | CA15a | F 5′-GTGAACGTTAAGGCGATCTACA-3′ | 131 | ENSGMOT00000007252 |
| R 5′-TAGTACTTGGTACGGTCCACTC-3′ | ||||
| Carbonic anhydrase 15b | CA15b | F 5′-GCTCCGAACACATGCTGAA-3′ | 120 | ENSGMOT00000002626 |
| R 5′-TGGTCAGGGAACCCATGTA-3′ | ||||
| REFERENCE GENES | ||||
| Ribosomal protein L4 | RPL4 | F 5′-CTCCACCATCAAGATCAACTAC-3′ | 160 | ENSGMOT00000009188 |
| R 5′-CTTCAGGTTCTTCAGAGGATTC-3′ | ||||
| Ubiquitin conjugated enzyme 2a | UCE2a | F 5′-CATATTTGGACCAGAGGGAAC-3′ | 152 | ENSGMOG00000001702 |
| R 5′-CTAAACATATGCTGCCATCGG-3′ | ||||
F, forward primer; R, reverse primer.