Table 3.

Analysis of variance (ANOVA) results of protein and mRNA concentrations of intestinal ion transporters under different pCO₂ conditions (pH 8.1; 550 μatm, pH 7.8; 1200 μatm and pH 7.6; 2200 μatm at the two temperatures of 10 and 18°C.

Protein	Two-way ANOVA				Comparisons for factor
	Treatment	df	F	P	Factor		t	P
NKA	pCO2	2, 25	1.202	0.317	18°C	8.1 vs. 7.6	3.770	0.003*
	T	1, 25	3.248	0.084		8.1 vs. 7.8	2.432	0.045*
	pCO2 × T	2, 25	7.909	0.002*		7.6 vs. 7.8	1.506	0.145
NBC	pCO2	2, 29	1, 178	0.322	10°C	7.6 vs. 8.1	3.045	0.015*
	T	1, 29	23, 16	<0.001*		7.6 vs. 7.8	2.768	0.019*
	pCO2 × T	2, 29	6, 270	0.005*		7.8 vs. 8.1	0.405	0.688
VHA	pCO2	2, 30	5, 383	0.010*	10°C	7.6 vs. 7.8	3.260	0.008*
	T	1, 30	6, 231	0.018*		7.6 vs. 8.1	1.758	0.170
	pCO2 × T	2, 30	4, 215	0.024*		8.1 vs. 7.8	1.501	0.144
					18°C	8.1 vs. 7.8	2.741	0.030*
						8.1 vs. 7.6	2.252	0.063
						7.6 vs. 7.8	0.489	0.628
SLC26A6	pCO2	2, 28	3, 176	0.057
	T	1, 28	0.013	0.912
	pCO2 × T	2, 28	1, 072	0.356
NHE3	pCO2	2, 28	1, 565	0.227
	T	1, 28	2, 833	0.103
	pCO2 × T	2, 28	1, 581	0.224
CLC3	pCO2	2, 28	1, 393	0.265
	T	1, 28	3, 856	0.060
	pCO2 × T	2, 28	0.285	0.754
mRNA
CA2a	pCO2	2, 24	13.61	0.006	18°C	8.1 vs. 7.8	9.089	<0.001*
	T	1, 24	9.173	<0.001*		8.1 vs. 7.6	9.079	<0.001*
	pCO2 × T	2, 24	15.26	<0.001*		7.6 vs. 7.8	0.011	1
CA2b	pCO2	2, 36	1.211	0.053
	T	1, 36	4.019	0.310
	pCO2 × T	2, 36	2.798	0.074
CA4b	pCO2	2, 32	8.367	0.361
	T	1, 32	0.859	0.001*
	pCO2 × T	2, 32	0.583	0.564
CA15	pCO2	2, 34	35.72	<0.001*	10°C	7.6 vs. 8.1	14.66	<0.001*
	T	1, 34	44.53	<0.001*		7.6 vs. 7.8	14.45	<0.001*
	pCO2 × T	2, 34	36.45	<0.001*		7.8 vs. 8.1	0.202	0.989
VHAa	pCO2	2, 36	1.011	0.374
	T	1, 36	0.159	0.693
	pCO2 × T	2, 36	1.520	0.232
ATP1A1	pCO2	2, 31	0.939	0.402	10°C	7.6 vs. 8.1	3.693	0.036*
	T	1, 31	10.27	0.003*		7.6 vs. 7.8	0.497	0.934
	pCO2 × T	2, 31	3.597	0.039*		7.8 vs. 8.1	3.335	0.063
NHE1a	pCO2	2, 37	0.734	0.487
	T	1, 37	3.110	0.086
	pCO2 × T	2, 37	0.500	0.611
NHE3	pCO2	2, 35	6.267	0.005*	10°C	7.6 vs. 7.8	7.099	<0.001*
	T	1, 35	15.15	<0.001*		7.6 vs. 8.1	5.792	<0.001*
	pCO2 × T	2, 35	8.039	0.001*		8.1 vs. 7.8	0.836	0.826
NBCa	pCO2	2, 35	7.144	0.003*	10°C	7.6 vs. 8.1	5.466	0.001
	T	1, 35	4.030	0.052		7.6 vs. 7.8	4.927	0.004
	pCO2 × T	2, 35	3.356	0.046*		7.8 vs. 8.1	0.745	0.859
NBCb	pCO2	2, 35	0.297	0.745
	T	1, 35	4.980	0.032*
	pCO2 × T	2, 35	0.832	0.444
26A3.2	pCO2	2, 28	5.317	0.011*	18°C	7.8 vs. 7.6	5.715	0.001*
	T	1, 28	1.451	0.238		7.8 vs. 8.1	3.325	0.065
	pCO2 × T	2, 28	5.875	0.007*		8.1 vs. 7.6	2.545	0.188
26A6a	pCO2	2, 31	6.029	0.006*	10°C	7.6 vs. 7.8	4.947	0.004*
	T	1, 31	0.024	0.878		7.6 vs. 8.1	4.582	0.008*
	pCO2 × T	2, 31	3.405	0.046*		8.1 vs. 7.8	0.574	0.914
Rhbg	pCO2	2, 30	6.417	0.005	18°C	7.6 vs. 8.1	6.080	<0.001*
	T	1, 30	6.736	0.014		7.6 vs. 7.8	6.277	<0.001*
	pCO2 × T	2, 30	6.416	0.005		7.8 vs. 8.1	0.011	1

^*Indicates significant (p < 0.05) differences.