Figure 2.

Effect of SbSLSP on stress tolerance in T₂ transgenic tobacco. (A,B) Germination of SbSLSP transgenic and wild-type plants in MS medium supplemented with 200 mM NaCl or 300 mM mannitol. The percentage of germinated seeds was calculated based on the number of seedlings that reached the cotyledon stage by 9 days. (C) Root growth comparisons of the germinated seedlings of transgenic and WT plants, which were grown on vertical plates for 10 days in MS media for control experiment and 21 days for MS media supplemented with 200 mM NaCl or 300 mM mannitol for stress experiment. (D,E) Graphical representation of root measurements of transgenic and wild-type plants in control and stress conditions (salt or drought stress), respectively. Seeds of each line were germinated and planted on MS agar, or MS agar supplemented with 200 mM NaCl or 300 mM mannitol in triplicate. Root length was measured at 10 days for MS agar and 21 days for MS agar supplemented with 200 mM NaCl or 300 mM mannitol. Values are the mean ± SD (triplicate measurements; n = 10). (F) Fresh weight measurement of 21-day-old seedlings in salt and drought stress. Values are the mean ± SD (triplicate measurements; n = 10).