Table 1.
Primer sequences for genes of interest
| Gene | Primer sequence (5′–3′) | Ref. sequence number | Amplicon length (bp) | GC% content |
|---|---|---|---|---|
| AR | F:GCCTCCGAAGTGTGGTATCC R:CCTGGTACTGTCCAAACGCA |
NM_013476.3 | 138 | 60 55 |
| IGF-IR | F:CTACCTCCCTCTCTGGGAATG R:GCCCAACCTGCTGTTATTTCT |
NM_010513 | 185 | 47.4 47.4 |
| MyoD | F:CATTCCAACCCACAGAAC R:GGCGATAGAAGCTCCAA |
NM_010866 | 125 | 59.7 50 |
| Myostatin | F:TACTCCGAATAGAAGCCATAA R:GTAGCGTGATAACGTCATC |
NM_010834 | 194 | 36.3 45 |
| Myogenin | F:CCAACTGAGATTGTCTGTC R:GGTGTTAGCCTTATGTGAAT |
NM_031189 | 173 | 47.3 40 |
| RP-IIb | F:GGTCAGAAGGGAACTTGTGGTAT R:GCATCATTAAATGGAGTAGCGTC |
NM_153798.1 | 197 | 50 44.4 |