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. 2016 Apr 5;6(6):1649–1659. doi: 10.1534/g3.116.029520

Figure 4.

Figure 4

Stress sensitive phenotypes caused by reduced expression of TTI2. (A) CY6070 (DED1-TTI2), CY6971 (GAL10-TTI2), and mec2-1 (included as a control for sensitivity to hydroxyurea; Weinert et al. 1994), were grown to stationary phase in YP medium containing raffinose then 10-fold serial dilutions spotted onto YP plates containing raffinose, or with raffinose, and either 0.1 M hydroxyurea, 6% ethanol, 8 μg/ml Calcofluor white, 1.0 nM rapamycin, or 1 M NaCl and grown for 3 d at 30° or 37°. (B) CY6070, CY6971, and CY6874 (tti2Q276TAA) were grown to stationary phase in YPD medium then 10-fold serial dilutions spotted onto YPD plates, or YPD with either 6% ethanol, 8 μg/ml Calcofluor white, 1.0 nM rapamycin, or 10 mM caffeine, and grown at 30° for 3 days.