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Localization of Tra1 and Mec1 upon depleting Tti2. Strains CY5998 (eGFP-TRA1 WT-TTI2), CY7193 (eGFP-TRA1 GAL10-TTI2), CY6306 (eGFP-MEC1 WT-TTI2), and CY7189 (eGFP-MEC1 GAL10-TTI2) were grown to stationary phase in synthetic complete medium containing raffinose, diluted 1:20 in synthetic complete medium containing glucose, grown to midlogarithmic phase, and stained with Hoechst stain. Cells were harvested and washed twice in sterile water, then imaged using fluorescence microscopy.
