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. 2016 Apr 6;6(6):1661–1671. doi: 10.1534/g3.116.028068

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Copyright © 2016 Paranjape and Calvi

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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H3.3 is not essential for genomic DNA replication or developmental gene amplification. (A)–(D) Follicle cell nuclear DNA labeled with DAPI (blue) and for EdU incorporation (green) during genomic replication (A and B) and amplification (C and D) in wild type control (A and C) or H3.3 null females (B and D). The H3.3 null females were homozygous mutant for an H3.3B null allele on the X, and a null H3.3A allele over deficiency on the second, H3.3B⁰; H3.3A^2*1 / Df(2L)BSC110. Scale bars are 10 µm.