Figure 5.

STAG3 is required for stable axial localization of meiosis-specific cohesins, but not the mitotic cohesins. (A–C) Example chromatin spread preparations from purified testicular germ cells of control, Rad21l, Rec8, Stag3 single mutants, and the three possible double mutant combinations aged 15 d postpartum. Chromatin spreads were immunolabeled using antibodies against the SC lateral element protein SYCP3 (red), and either SMC3 (A), or SMC1α (B), or SMC1β (C), all of which are shown in green. Zygotene and pachytene stages are depicted for control, and typical examples for each mutant are given. The numbers within each chromatin spread represent the average Manders’ colocalization/overlap coefficient for each cohesin component within the SYCP3 axes (N = 25 chromatin spreads per strain). Scale bars = 10 µm.