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CRISPR/Cas9-mediated gene deficiency in chicken ESCs. (A) Effect of CRISPR/Cas9 plasmid transfection into chicken ESCs. Right: results of the T7EI assay indicate C2EIP gene knockout. (B) Results of qPCR and Western blot analysis show downregulated expression of the C2EIP gene and protein (** P < 0.01).
