Figure 6.

Effect of DFMO, licofelone, and the combination on cell proliferation markers in NNK-induced lung tumors from mice in different treatment groups. A. Representative western blot of PCNA and Cyclin D1 measured in total cell protein of tumor. B. Relative mRNA expression of PCNA in RNA samples from different treatment groups as analyzed by real-time PCR and normalized to β-actin. C. Representative photomicrographs showing immunohistochemical detection of PCNA, Cyclin D1, and Cyclin A (magnification ×600; inset shows 400× magnification). Differences between control and treatment groups were analyzed by one-tailed t-test with Welch correction and 95% confidence interval. Data with means ± SE. p ≤ 0.05 was considered statistically significant.