Figure 1.

rL-RVG induces the expression of ER stress-related proteins in SGC-7901 and HGC cells in a time-dependent manner. A and D. For SGC; B and E. For HGC. The cells were infected with 10 MOI virus or with vehicle (control) for different amounts of time. Cell lysates were harvested at the same time after infection with virus for different amounts of time, and proteins were detected by western blotting. A and D. The expression of GRP78 and CHOP increased at 12 h after treatment with rL-RVG, peaked at 24 h (**, p<0.01) and was sustained for at least 12 h in SGC cells. The same was observed in B and E. for HGC cells. A transient increase in eIF2α phosphorylation following rL-RVG infection was observed at 24 h in SGC cells (**, p<0.01) and at 12 h in HGC cells (**, p<0.01). C. Effect of rL-RVG on the level of spliced mRNA forms of XBP-1. uXBP1, unspliced XBP-1; sXBP-1, spliced XBP-1. The spliced XBP-1 mRNA appeared with 12 h of pretreatment, but the effect was transient.