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. 2016 May 1;6(5):924–936.

Figure 4.

Figure 4

N+4: NDV+4-PBA; R+4: rL-RVG+4-PBA. The contribution of ER stress downregulation to the expression of autophagy- and apoptosis-associated proteins in SGC and HGC cells after infection. A. For SGC; B. For HGC. The expression of the autophagy-associated proteins beclin-1 and LC3-II/I decreased in cells with 4-PBA treatment compared with cells without 4-PBA treatment. The same was observed for apoptosis-associated proteins. C and D. Early apoptotic (annexin V+/PI-) and late apoptotic (annexin V+/PI+) cells. Prior to fluorescence-activated cell sorting analysis of annexin V/PI staining, SGC cells were pretreated with 4-PBA (10 mM) 2 h before exposure to NDV or rL-RVG (10 MOI). Annexin V/PI staining was then performed to assess apoptosis/necrosis. Annexin V staining showed that pretreatment with 10 mM 4-PBA resulted in a significant decrease in apoptosis in the rL-RVG-infected SGC cells (**, p<0.01).