Table 1.
Efficacy of Anti-RON ADC Zt/g4-DM1 in vivo in Regulating Cell Cycle, Viability, and Death of Pancreatic Cancer Cell lines*
| Human PDAC cell lines | RON receptor per cell | IC50 values of free DM1 (nM) | EC50 values of Zt/g4-DM1 in PDAC cells | |||
|---|---|---|---|---|---|---|
|
| ||||||
| Endocytosis of RON (h) | G1/G0 Phase Change (h) | Cell Viability (µg/ml) | Cell Death (µg/ml) | |||
| Panc-1 | < 100 | 6.64 ± 1.07 | ND | ND | > 100 | ND |
| BxPC-3 | 10,214 ± 310 | 2.07 ± 0.37 | 12.77 | 10.33 | 2.33 ± 0.35 | 5.63 ± 0.21 |
| FG | 16,178 ± 269 | 2.25 ± 0.24 | 17.22 | 14.04 | 3.34 ± 0.42 | 3.27 ± 0.43 |
| L3.6pl | 16,628 ± 245 | 4.69 ± 0.64 | 14.14 | 13.75 | 3.02 ± 0.45 | 2.24 ± 0.18 |
| Average | 14,340 ± 3580 | 3.91 ± 1.47 | 14.71 ± 2.28 | 12.71 ± 2.06 | 2.95 ± 1.03 | 3.37 ± 1.34 |
*
Individual PDAC cell lines were used to determine RON expression, cellular sensitivity to free DM1, Zt/g4-DM1-induced RON internalization, cell cycle change, cell viability, and cell death as detailed in Materials and Methods.