Table 2.
Induction of cell cycle changes by anti-RON ADC Zt/g4-DM1 in pancreatic cancer cell lines*
| PDAC cell lines | Changes of cells cycles after Zt/g4-dM1 treatment (%) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
||||||||||||||
| G0/G1 phase | S phase | G2M phase | Polyploidy | |||||||||||
|
|
||||||||||||||
| 0 h | 12 h | 24 h | 36 h | 0 h | 12 h | 24 h | 36 h | 0 h | 12 h | 24 h | 36 h | 0 h | 36 h | |
| L3.6pl | 45.24 | 33.91 | 8.55 | 7.59 | 23.25 | 26.01 | 16.44 | 22.45 | 23.58 | 34.01 | 53.34 | 54.05 | 7.93 | 14.95 |
| BxPc-3 | 44.58 | 24.55 | 7.05 | 7.03 | 21.31 | 14.09 | 16.00 | 15.73 | 31.51 | 50.06 | 61.39 | 62.36 | 2.61 | 14.85 |
| FG | 42.23 | 31.73 | 26.01 | 22.48 | 21.92 | 20.65 | 18.15 | 17.14 | 27.49 | 36.85 | 46.31 | 50.74 | 8.36 | 9.64 |
| Average | 44.02 ± 1.58 | 30.06 ± 4.90 | 13.87 ± 10.5 | 12.16 ± 8.76 | 22.16 ± 0.99 | 20.25 ± 5.97 | 16.86 ± 1.14 | 18.44 ± 3.54 | 27.53 ± 3.97 | 40.31 ± 8.57 | 53.68 ± 7.55 | 55.73 ± 6.00 | 6.30 ± 3.20 | 13.15 ± 3.04 |
*
Individual PDAC cell lines were treated with 5 µg/ml of Zt/g4-DM1 for various time intervals followed by flow cytometric analysis to determine their cell cycles as previously described [16].