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. 2016 May 1;6(5):981–995.

Figure 5.

Figure 5

GLUT4 is a key executor of HER2-driven ADH-like acini progression to DCIS-like acini. A. qRT-PCR analysis of GLUT4 mRNA expression in 10A.B2 cells transfected with control siRNA (Ctrl) or GLUT4 siRNA (siGLUT4) four days after transfection. B. Western blotting analysis of GLUT4 protein level in indicated cells as in A. C. Proliferation assay for indicated cells as in A measured by MTT assay at 0, 24, 48 and 72 hours. D. Immunofluorescence staining analysis (on day 12 of 3D culture) of Ki-67 and cleaved caspase-3 in 10A.B2 cells transfected with siCtrl or siGLUT4 on day 0. Ki-67 and cleaved caspase-3 are shown in green. Laminin V is shown in red. DAPI is shown in blue. Scale bar represents 50 µm. Light microscopy photos (top panel) are used as reference. Scale bar represents 100 µm for light microscopy images. E. Glucose uptake assay of indicated cell lines. F. Lactate production assay of indicated cell lines. G. Oxygen consumption assay of indicated cell lines. n.s., not significant, *, P < 0.05, **, P < 0.01 and ***, P < 0.001.