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. 2016 May 1;6(5):996–1010.

Figure 6.

Figure 6

Combining siYAP/TAZ with metformin treatment completely blocked the effects of insulin on cell proliferation. A. Cell proliferation was inhibited by metformin in a dose dependent manner. KLE cells were treated with metformin at 0.5 μM, 1 μM, 2 μM and 4 μM. After 48 h treatment, cell viability was measured by SRB. Statistical significance was calculated using two way ANOVA and Sidak’s multiple comparisons test, ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.5, ns means non-significant. Error bars represent Mean ± SD of triplicates. B. EFE184 were treated in a same way like KLE. Cell viability was measured by SRB. C. EFE184 were treated metformin and insulin with the according dose described in the figure. After 48 h treatment, Cell viability was measured by SRB. D. EFE184 cells were transfected with siYAP and siTAZ. After 24 h, cells were administrated with Insulin (10 ng/ml) and metformin with the dose as shown in the figure. After 48 h, cells were processed to SRB assay to test the cell proliferation.